Changes in Spermatogenesis, Lipoperoxidation Processes, and Antioxidant Protection in Men with Pathozoospermia after COVID-19. The Effectiveness of Correction with a Promising Antioxidant Complex.

The indicators of spermatogenesis and the state of LPO and antioxidant protection in men with pathozoospermia after COVID-19 were assessed before and after treatment an antioxidant complex. Blood plasma served as the material for biochemical studies. In the examined patients, the parameters of spermatogenesis, as well as blood concentration of LPO components (diene conjugates and TBA-reactive substances) were analyzed. The total antioxidant activity of the blood was determined as an indicator characterizing the total activity of LPO inhibitors and determining its buffer capacity. In patients recovered from COVID-19, an increase in spermatogenesis disorders and shifts towards the predominance of prooxidant factors were observed. After a course (1 month) of antioxidant complex, patients showed increased sperm motility, decreased leukocyte count in the ejaculate, and restored balance in the prooxidant-antioxidant system towards antioxidant components. The effectiveness of correction of post-COVID disorders largely depends on the degree of damage to the structure and function of cell membranes caused by oxidative stress. The use of the antioxidant complex is a promising option, because it reduces the level of LPO, enhances antioxidant protection of the body, and also normalizes some parameters of spermatogenesis.

Chemiluminescence analysis of free radical processes in seminal plasma of men with pathospermia who have undergone COVID-19 infection

Introduction. In the context of the COVID-19 pandemic, it seems relevant to study the clinical features and the effect of the SARS-CoV-2 virus on the reproductive system and male fertility. The study objective was to assess the intensity of oxidative processes in the seminal plasma of men with pathozoospermia after suffering a COVID-19 infection. Materials and methods. Spermogram parameters were assessed in accordance with the criteria of the 2010 WHO Guidelines, 5th edition, for the study and processing of human ejaculate two to three months after the patient received a negative polymerase chain reaction result of a nasopharyngeal swab for SARS-CoV-2 DNA. The intensity of free radical processes in seminal plasma was investigated by the method of chemiluminescence induced by ions of bivalent iron. Results. A decrease in sperm motility and an increase in the number of leukocytes were found in men with pathozoospermia who had undergone COVID-19 infection. There are lower levels of indicators such as ejaculate volume, total sperm concentration and the number of morphologically normal sperm forms. An increased ability to generate reactive oxygen species in seminal plasma was revealed in pathozoospermia and COVID-19. Conclusions. The results obtained indicate that SARS-CoV-2 affects the male reproductive system directly or indirectly and has a negative effect on male reproductive health, causing the activation of lipid peroxidation processes and spermatogenic insufficiency. © 2021 Cyrillo-Methodian Research Centre of BAS. Anales de Investigación,. All rights reserved.

[Male reproductive health and COVID-19]

The SARS-CoV-2 pandemic has brought serious economic and social problems worldwide'. Due to its medical consequences, it is of importance to study the mechanisms of the disease and new therapeutic interventions, as well as rehabilitation processes. Despite the fact that the genome of the new coronavirus has been sequenced and studied, clinical and epidemiological data are constantly updated and analyzed, and exact pathogenesis has not yet been understood. At the same time, domestic and foreign studies suggest that the virus is an agent that affects not only the lungs, vascular wall, hemostasis, but also the reproductive system. The aim of the review is to summarize the current knowledge about novel SARS-CoV-2, including its pathophysiology and potential impact on male reproductive function.

Glutathione-dependent mechanisms of antioxidant defense in men with pathozoospermia after covid-19 infection

The article presents results of studying the influence of COVID-19 infections on glutathione metabolism in men with pathozoospermia and COVID-19. In erythrocyte cytolysate, we determined concentration of reduced (GSH) and oxidized (GSSG) glutathione and the GSH/GSSG ratio as the main indication of cell redox-status and an important factor in cell redox-dependent signaling control along with the effect GSH-dependent enzymes. Results of our study allowed us to determine the peculiarity of the influence of COVID-19 infection on thee glutathione metabolic pathway. The understanding of the influence mechanisms of SARS-CoV-2 on metabolic processes in the body of men with pathozoospermia has been expanded. The data obtained give grounds and opportunities for a more reasonable approach to the organization of preventive, curative, and rehabilitative measures for COVID-19 infection. © 2021, International Medical Research and Development Corporation. All rights reserved.